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Identification of Berberis spp. as alternate hosts for Puccinia achnatheri-sibirici under controlled conditions

作者:   来源:   发布日期:2020-06-26  浏览次数:

论文信息:  Identification of Berberis  spp. as alternate hosts for   Puccinia achnatheri-sibirici  under controlled conditions and morphologic observations of sexual stage development of the rust fungus. Xinyao Ma, Yao Liu, Qiao Li, Xiaxia Tian, Zhimin Du, Zhensheng Kang*, Jie Zhao*. Froniters in Microbiology. 2020, 11,1278.

JCR分区Q1,中科院大类分区2区,IF=4.259

论文摘要:Gramineous grasses are a large group of species, many of which act as accessory (secondary) host for a large number of rust fungi, including devastating rust pathogens of cereals. Among the rust fungi, some are known to be heteroecious and have a complete macrocyclic lifecycle with five types of spores on distinct plant species, but for many others the complete life cycle is unknown.   Puccinia achnatheri-sibirici , a rust fungus infecting grasses in the genus   Achnatherum , has been known for only its uredinial and telial stages, however the other (pycnial and aecial) stages has not been identified. In this study, we demonstrate that   P. achnatheri-sibirici is a heteroecious, macrocyclic fungus with the sexual stage on barberry (  Berberis spp.) through inoculation Pycnia and aecia were successively produced on the inoculated barberry plants. Inoculation of  Achnatherum extremiorientale leaves with aeciospores produced yellow-orange uredinia with high infection types, whereas inoculation of wheat variety Mingxian 169, highly susceptible to   Puccinia striiformis f. sp.  tritici  which causes stripe rust on wheat, produced chlorotic flecks but no uredinia. The ITS sequence analysis of   P. achnatheri-sibirici  did not match with any sequence in the NCBI database, and had the highest homology with 94% compared to  Pucciniabrachypodii and Puccinia aizazi . Observations of the uredinial, telial, and basidial stages on A. extremiorientale and pycnial and aecial stages on  Berberis shensiana using light and scanning electron microscopes revealed its characteristics. Morphological characteristics of urediniospores and teliospores are most similar with those of  P. achnatheri-sibirici described in the literature. This study proved that (1) the life cycle of  P. achnatheri-sibirici  as heteroecious and macrocyclic and the alternate host as barberry;(2) the description of life stages in the sexual cycle, especially the morphologies of aecial, pycnial and basidial stages;and (3) the expansion of knowledge on the rust flora on barberry.

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