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A small cysteine-rich protein from two kingdoms of microbes is recognized as a novel pathogen-associated molecular pattern

作者:   来源:   发布日期:2019-04-28  浏览次数:

       论文信息:Jiajun Nie , Zhiyuan Yin , Zhengpeng Li, Yuxing Wu and Lili Huang. A small cysteine-rich protein from two kingdoms of microbes is recognized as a novel pathogen-associated molecular pattern. New Phytologist (2019) 222: 995–1011.

       JCR分区Q1,中科院大类分区一区,IF=7.433

        论文摘要:Pathogen-associated molecular patterns (PAMPs) are conserved molecules that are crucial for normal life cycle of microorganisms. However, the diversity of microbial PAMPs is little known. During screening of cell-death-inducing factors from the necrotrophic fungus Valsa mali, we identified a novel PAMP VmE02 that is widely spread in oomycetes and fungi.Agrobacterium tumefaciens-mediated transient expression or infiltration of recombinant protein produced by Escherichia coli was performed to assay elicitor activity of the proteins tested. Virus-induced gene silencing in Nicotiana benthamiana was used to determine the components involved in VmE02-triggered cell death. The role of VmE02 in virulence and coni-diation of V.mali were characterized by gene deletion and complementation.We found that VmE02, together with some of its homologues from both oomycete and fungal species, exhibited cell-death-inducing activity in N. benthamiana. VmE02-triggered cell death was shown to be dependent on BRI1-ASSOCIATED KINASE-1, SUPPRESSOR OF BIR1-1, HSP90 and SGT1 in N.benthamiana. Deletion of VmE02 in V.mali greatly attenuated pathogen conidiation but not virulence, and treatment of N.benthamiana with VmE02 enhances plant resistance to Sclerotinia sclerotiorum and Phytophthora capsici. We conclude that VmE02 is a novel cross-kingdom PAMP produced by several fungi and oomycetes.

 

 

 

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